Biotechnology Advances Impact Factor

June 27, 2015
On Biotechnological

Cover imageSystematic review about advances and perspectives for UGT mediated glycosylation

Synthases and phosphorylases emerge as novel route for in vivo UDP-sugar generation.

Glycorandomization fuels after that generation glycosides.

Metabolic manufacturing unlocks de novo production as alternative to removal.

Glycosylation of small molecules like specific (additional) metabolites features a profound affect their particular solubility, stability or bioactivity, making glycosides appealing compounds as food additives, therapeutics or nutraceuticals. The subsequently developing marketplace demand features fuelled the development of different biotechnological procedures, and this can be divided within the inside vitro (using enzymes) or in vivo (using whole cells) creation of glycosides. Inside context, uridine glycosyltransferases (UGTs) have emerged as promising catalysts the regio- and stereoselective glycosylation of various tiny molecules, hereby using uridine diphosphate (UDP) sugars as activated glycosyldonors. This analysis provides a comprehensive summary of the recently developed in vivo production processes making use of UGTs and covers the main paths towards UDP-sugar formation. Moreover, using interconverting enzymes and glycorandomization is highlighted when it comes to production of uncommon or new-to-nature glycosides. Eventually, the technical difficulties and future trends in UDP-sugar based glycosylation tend to be critically assessed and summarized.

Keywords

  • Glycosylation;
  • Tiny molecules;
  • UDP-sugars;
  • Glycosyltransferases;
  • in vivo;
  • In vitro;
  • Glycorandomization;
  • Glycosides;
  • UGT;
  • Metabolic manufacturing
Source: www.sciencedirect.com
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