Biotechnology Letters Journal

January 22, 2015
Marrone Bio Innovations

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Objective

The Δ9-tetrahydrocannabinolic acid synthase (THCAS) from Cannabis sativa ended up being expressed intracellularly in various organisms to analyze the potential of a biotechnological creation of Δ9-tetrahydrocannabinolic acid (THCA) using entire cells.

Results

Functional appearance of THCAS had been acquired in Saccharomyces cerevisiae and Pichia (Komagataella) pastoris using a signal peptide from vacuolar protease, proteinase A. No useful appearance ended up being accomplished in Escherichia coli. The greatest volumetric activities gotten were 98 pkat ml−1 (intracellular) and 44 pkat ml−1 (extracellular) after 192 h of cultivation at 15 °C making use of P. pastoris cells. Low solubility of CBGA prevents the THCAS application in aqueous cell-free systems, thus entire cells were utilized for a bioconversion of cannabigerolic acid (CBGA) to THCA. Eventually, 1 mM (0.36 g THCA l−1) THCA might be made by 10.5 gCDW l−1 before enzyme activity was lost.

Conclusion

Entire cells of P. pastoris deliver capacity for synthesizing pharmaceutical THCA manufacturing

Keywords

Cannabigerolic acid Cannabis sativa Pichia pastoris Δ9-Tetrahydrocannabinolic acid Synthase Whole mobile bioconversion See also:
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Source: link.springer.com
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