Extraction of glycosides

August 29, 2017
Patent EP2642859A2 - Method of

Cover imagean extraction method of steviol glycosides from stevia leaves had been optimised.

The removal technique set up is fast and modified for stevia genotype assessment.

The experimental results gave a maximal removal yield.

Following approval of steviol glycosides as a food additive in Europe in December 2011, large-scale stevia cultivation should be created inside the EU. Hence discover a need to increase the efficiency of stevia analysis through germplasm enhancement and agronomic enhancement programs. To deal with the need for quicker and reproducible test throughput, problems for automated removal of dried stevia departs utilizing Accelerated Solvent Extraction were optimised. A response area methodology was familiar with research the influence of three facets: removal heat, static some time pattern quantity from the stevioside and rebaudioside A extraction yields.

The model indicated that all facets had an individual impact on the yield. Maximum removal circumstances had been set at 100 °C, 4 min and 1 cycle, which yielded 91.8percent ± 3.4percent of complete extractable steviol glycosides analysed. One More optimisation was achieved by decreasing the grind measurements of the leaves offering your final yield of 100.8% ± 3.3%.

Keywords

  • Stevia rebaudiana Bertoni;
  • Accelerated Solvent Extraction;
  • Steviol glycosides;
  • Genotype assessment;
  • Response surface methodology;
  • HPLC
Source: www.sciencedirect.com
RELATED VIDEO
Admission Notice- Department of Biotechnology Announces
Admission Notice- Department of Biotechnology Announces ...
Promotional video of Department of Biotechnology, Osaka
Promotional video of Department of Biotechnology, Osaka ...
Department of Molecular Biotechnology and Bioinformatics
Department of Molecular Biotechnology and Bioinformatics ...
RELATED FACTS
Share this Post
latest post
Tweets