Glycoside hydrolases

June 25, 2018
Glycoside hydrolases encoded

Typically, the hydrolysis regarding the glycosidic relationship is catalyzed by two amino acid residues of this enzyme: an over-all acid (proton donor) and a nucleophile/base . With regards to the spatial position of these catalytic deposits, hydrolysis occurs via overall retention or general inversion of the anomeric configuration. For every family members listed in the CAZy database, we suggest (when it's understood) the stereochemical outcome of the response catalyzed as well as the variety of amino-acid deposits acting as a nucleophile/base and as a proton donor. In many cases, the catalytic nucleophile is not borne by the enzyme, and it is changed because of the acetamido team at C-2 of the substrate . A completely unrelated procedure has-been demonstrated recently for just two categories of glycosidases utilizing NAD+ as a cofactor .

For a detailed description for the catalytic system (and variants thereof) of glycoside hydrolases, kindly see .

Tables for Direct Access

GH Family Number

GH Classification Statistics

Modules in current people 279221
Non-Classified segments 4109

GH Clans of Related Families

5-fold β-propeller

EC strategies within GH households

Care : due to the standard nature of CAZymes, these activities might not be straight from the family but merely borne by adjacent modules.


Henrissat B (1991) a category of glycosyl hydrolases centered on amino-acid series similarities. Biochem. J. 280:309-316 [PMID: 1747104].

Henrissat B, Bairoch A (1993) brand new families inside category of glycosyl hydrolases according to amino- acid series similarities. Biochem. J. 293:781-788 [PMID: 8352747].

Henrissat B, Bairoch the (1996) Updating the sequence-based classification of glycosyl hydrolases. Biochem. J. 316:695-696 [PMID: 8687420].

Davies G, Henrissat B (1995) Structures and mechanisms of glycosyl hydrolases. Construction 3:853-859 [PMID: 8535779].

Terwisscha van Scheltinga A, Armand S, Kalk KH, Isogai the, Henrissat B, Dijkstra BW (1995) Stereochemistry of chitin hydrolysis by a plant chitinase/lysozyme and x-ray structure of a complex with allosamidin. Research for substrate assisted catalysis. Biochemistry 34:3
[PMID: 7495789].

Rajan SS, Yang X, Collart F, Yip VL, Withers SG, Varrot the, Thompson J, Davies GJ, Anderson WF (2004) Novel catalytic process of glycoside hydrolysis on the basis of the construction of an NAD+/Mn2+ -dependent phospho-alpha-glucosidase from Bacillus subtilis. Structure. 12:1619-1629
[PMID: ].

Liu QP, Sulzenbacher G, Yuan H, Bennett EP, Pietz G, Saunders K, Spence J, Nudelman E, Levery SB, White T, Neveu JM, Lane WS, Bourne Y, Olsson ML, Henrissat B, Clausen H (2007) Bacterial glycosidases when it comes to production of universal purple bloodstream cells. Nature Biotechnol. 25:454-464

Carbohydrate glycoside formation/hydrolysis
Carbohydrate glycoside formation/hydrolysis
Chem 112C - Glycoside Formation - Mechanism
Chem 112C - Glycoside Formation - Mechanism
Formation of a glycosidic bond. Alpha glucose
Formation of a glycosidic bond. Alpha glucose
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