Nature Biotechnology 18, 645 - 648 (2000)
Surface-displayed viral antigens on Salmonella company vaccine
Jong-Soo Lee, Kwang-Soon Shin, Jae-Gu Pan & Chul-Joong Kim
We've created a recombinant real time oral vaccine utilizing the ice-nucleation necessary protein (Inp) from Pseudomonas syringae to display viral antigens at first glance of Salmonella spp. Fusion proteins containing viral antigens had been expressed into the dental vaccine strain, Salmonella typhi Ty21a. Surface localization ended up being verified by immunoblotting and fluorescence-activated cell sorting. The immunogenicity of surface-displayed viral antigens regarding recombinant real time vaccine strain had been examined in mice inoculated intranasally and intraperitoneally. Inoculation triggered considerably greater serum antibody level than those caused by viral antigens expressed intracellularly. Hence, this multivalent mucosal real time vaccine may possibly provide a successful method for inducing mucosal or systemic protected responses against numerous viral antigens.