Plant Biotechnology topics

September 24, 2016
Should we redesign humans?

The main objective regarding the division of Plant Biotechnology could be the development and constant enhancement of modern molecular biology approaches to application to analyze issues from area of plant cell biology and plant molecular genetics.

Plant photobiology

The primary study location is plant photobiology, particularly the performance of photoreceptors in addition to systems of light signal transduction. Current analysis subjects tend to be:

1. The performance of plant short-wave light photoreceptors – phototropins and UV receptors:

  • determining photoreceptors active in the regulation of gene phrase, analysis of phototropin gene expression at mRNA and protein level, elucidating the part of different A. thaliana phototropin splice variations, identifying proteins getting together with phototropins making use of bimolecular fluorescence complementation (BIFC) and yeast two-hybrid system
  • distinguishing 2nd messengers of short-wave light indicators and investigating the contacts between them: distinguishing phosphoinositides involved in blue light sign transduction and identifying their part in regulating the alterations in intracellular calcium focus, characterising different facets of the part of calcium as an additional messenger of light signals - experiments including the utilization of transgenic flowers carrying an inducible gene for aequorin connected to GFP including X-ray microanalysis, elucidating the role of endocytosis inside transduction of signals managing chloroplast motions, seeking indicators via the organelles themselves.
  • Investigating cross-talk between signaling paths; the modulation of light sign transduction by sugars and phytohormones

2. Components of light-induced intracellular movements:

Our research in this subject, in line with the style of chloroplast motions, includes: attempts to establish a connection between mobile polarity and directional motion process during leaf muscle regeneration, characterizing hawaii of proteins from the chloroplast envelope and their communications utilizing i.a. atomic force microscopy (AFM), examining the photoinduced alterations in communications between actin and myosin at first glance of remote chloroplasts, analyzing the functioning regarding the cytoskeleton in homozygous Arabidopsis thaliana T-DNA mutants with insertions in myosin genes, deciding the potential part of chloroplast motions in security of DNA against UV-induced harm.

3. The part of phytochromes in germination procedures

Two experimental models are utilized in this area of analysis: turions of Spirodela polyrhiza and seeds/seedlings of Solanum lycopersicum. The research jobs feature: determining the phytochromes which absorb light managing germination and storage product degradation, examining the metabolism of storage space products and its regulation by light, at the biochemical and molecular degree.

Source: www.uj.edu.pl
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